Effect of Sapindus Mukorossi Saponin Fraction and Anthraquinone Fraction of Rheum Emodi on Ccl4 Induced Cytochrome P450 Damage in Wistar Rats

The cytochrome P450 protective activity of Sapindus mukorossi and Rheum emodi were evaluated against CCl4 induced prolongation of pentobarbitone sleep time in Wister rats. The fractions, at a dose of 0.2 g/kg,0.4g/kg p.o., Sapindus mukorossi, Rheum emodi significantly prevents the CCl4 induced prolongation of pentobarbitone sleep time (P<0.01), indicating the cytochrome P450 protection activity. However, when treated alone, the fractions (0.2 g/kg and 0.4 g/kg, p.o.) shows a significant prolongation of pentobarbitone sleep time (P<0.01), indicating the intrinsic cytochrome P450 inhibition activity. In addition, the extracts show neither stimulant nor depressant effect on the CNS, as evident from locomotor activity test. In conclusion, the results indicate that, the Sapindus mukorossi saponin fraction and anthraquinone fraction of rheum emodi at an oral dose of 0.2 g/kg and 0.4 g/kg, p.o., shows significant protective effects against CCl4 induced cytochrome P450 damage and also show a significant intrinsic cytochrome P450 inhibition activity.